Isolation of Common Light Chain Antibodies from Immunized Chickens Using Yeast Biopanning and Fluorescence‐Activated Cell Sorting
نویسندگان
چکیده
منابع مشابه
Expression of heavy chain‐only antibodies can support B‐cell development in light chain knockout chickens
Since the discovery of antibody-producing B cells in chickens six decades ago, chickens have been a model for B-cell development in gut-associated lymphoid tissue species. Here we describe targeting of the immunoglobulin light chain locus by homologous recombination in chicken primordial germ cells (PGCs) and generation of VJCL knockout chickens. In contrast to immunoglobulin heavy chain knocko...
متن کاملApplication Note ► Purifying common light-chain bispecific antibodies using MCSGP
In this application note the purification of a common light-chain bispecific antibody using Multicolumn countercurrent solvent gradient purification (MCSGP) is described. The KNAUER Contichrom® system that is offered in cooperation of KNAUER and Chromacon is the only system on the market that can be used in MCSGP mode for difficult bioseparations. MCSGP is a countercurrent chromatographic proce...
متن کاملComparison of Four Different Purification Methods for Isolation of Anti Echis carinatus Antivenom Antibodies from Immunized Chicken Egg Yolk
Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...
متن کاملNeed for speed – finding productive mutations using transcription factor-based biosensors, fluorescenceactivated cell sorting and recombineering
The establishment of a bioeconomy requires technologies enabling the rapid development of microbial production strains and enzymes as highly efficient biocatalysts for the conversion of substrates derived from renewable carbon sources into a multitude of chemicals. For strain development, two major strategies are available. The classical approach uses several rounds of random mutagenesis and sc...
متن کاملIsolation and Identification of Yeast Strains Capable of Producing Single Cell Protein from Whey in Co-Cultures with Saccharomyces cerevisiae
In this study, twenty-five whey samples collected from dairy industries in the city of Isfahan. The sampleswere cultured on malt extract broth (MEB) and yeast extract glucose chloramphenicol agar (YGCA) media.Eleven yeast strains (designated M1 to M11) were isolated from the culture. The strains were identified bytheir morphological and physiological properties. Betagalactosid...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology Journal
سال: 2020
ISSN: 1860-6768,1860-7314
DOI: 10.1002/biot.202000240